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facs buffer with dapi  (Thermo Fisher)


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    Thermo Fisher facs buffer with dapi
    Facs Buffer With Dapi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/facs+buffer+dapi/dapi/pmc12036604-901-18-27
    Average 90 stars, based on 1 article reviews
    facs buffer with dapi - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    FACS:

    Article Title: Autophagy Is Dispensable for Macrophage-Mediated Lipid Homeostasis in Adipose Tissue
    Article Snippet: Fluorophore-conjugated antibodies were added for 30 min at 4°C with rotation, as follows: anti-CD45.2-Percp-Cy5.5 (1:100, v/v) immune cell marker (BD Biosciences), anti-F4/80-APC (1:20, v/v) macrophage marker (AbD Serotec), anti-CD11b (1:100, v/v) macrophage marker (Invitrogen), anti-CD11c-PE-TR (1:100, v/v), ATM marker (Invitrogen), and 4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene (BODIPY 493/503)–FITC (1:300 v/v) lipid marker (Invitrogen). .. Cells were resuspended in FACS buffer with DAPI (Invitrogen) and analyzed using an LSRII Flow Cytometer (Becton Dickson). .. Data analysis was performed with FlowJo software.

    Article Title: Myh11+ microvascular mural cells and derived mesenchymal stem cells promote retinal fibrosis
    Article Snippet: .. Collected cells were resupsended in FACS Buffer consisting of DMEM, 50% BSA, 5 mM EDTA, and DAPI (ThermoFisher, D1306, 1:1000 dilution). .. Myh11+ mural cells were FAC-sorted using a BD Influx Cell Sorter.

    Article Title: Reciprocal regulation of TLR4, TLR3 and Macrophage Scavenger Receptor 1 regulates nonopsonic phagocytosis of the fungal pathogen Cryptococcus neoformans
    Article Snippet: .. After staining, samples were resuspended in FACS buffer for single staining and unstained controls and FACS buffer with DAPI [ThermoFisher], a live dead stain, for all other samples. .. Stained samples were run on the Attune NxT flow cytometer [ThermoFisher] and acquired using the Attune NxT software [ThermoFisher].

    Article Title: Myh11 Lineage Corneal Endothelial Cells and ASCs Populate Corneal Endothelium
    Article Snippet: .. The remaining SVF was filtered through a 70-μm and 40-μm mesh, and later resuspended in FACS Buffer consisting of DMEM, 50% BSA, 5 mM EDTA, and DAPI (ThermoFisher, D1306, 1:1,000 dilution). .. Myh11-Lin(+) vSMC-PCs were FAC-sorted using a BD Influx Cell Sorter based on YFP expression, and immediately cultured into wells at an initial density of 1.5e4 cells/cm2.

    Article Title: Non-cell-autonomous tumor suppression by p53
    Article Snippet: Antibodies used for cell type identification were CD45-APC (1:500; BD Biosciences Pharmingen), Gr1-FITC (1:500; BD Biosciences Pharmingen) and Cd11b-PE (1:500; BD Biosciences Pharmingen). .. FACS buffer with DAPI (Invitrogen) was then added to each sample, followed by acquisition and sorting on an Aria1 sorter (BD). ..

    Flow Cytometry:

    Article Title: Autophagy Is Dispensable for Macrophage-Mediated Lipid Homeostasis in Adipose Tissue
    Article Snippet: Fluorophore-conjugated antibodies were added for 30 min at 4°C with rotation, as follows: anti-CD45.2-Percp-Cy5.5 (1:100, v/v) immune cell marker (BD Biosciences), anti-F4/80-APC (1:20, v/v) macrophage marker (AbD Serotec), anti-CD11b (1:100, v/v) macrophage marker (Invitrogen), anti-CD11c-PE-TR (1:100, v/v), ATM marker (Invitrogen), and 4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene (BODIPY 493/503)–FITC (1:300 v/v) lipid marker (Invitrogen). .. Cells were resuspended in FACS buffer with DAPI (Invitrogen) and analyzed using an LSRII Flow Cytometer (Becton Dickson). .. Data analysis was performed with FlowJo software.

    Staining:

    Article Title: Reciprocal regulation of TLR4, TLR3 and Macrophage Scavenger Receptor 1 regulates nonopsonic phagocytosis of the fungal pathogen Cryptococcus neoformans
    Article Snippet: .. After staining, samples were resuspended in FACS buffer for single staining and unstained controls and FACS buffer with DAPI [ThermoFisher], a live dead stain, for all other samples. .. Stained samples were run on the Attune NxT flow cytometer [ThermoFisher] and acquired using the Attune NxT software [ThermoFisher].



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    Image Search Results


    A Immunostaining of sagittal sections from a CS17 human embryo. Co-expression of NR2F2 (green) and SOX18 (blue) in the gut. scale bars 50 μm. B-B ”,and C-C ” Sections of proband liver (B) and duodenum (C) stained for CD31 (cyan), SOX18 (red arrows), NR2F2 (green arrows), and DAPI (purple). Orange and gold boxes denote zoomed in regions of individual vessels. Orange box represents vessel that shows cells co-expressing both NR2F2 and SOX18 in the same nucleus, whereas the gold box is a vessel with cells either expressing NR2F2 or SOX18. Scale bar = 50μm (B-C) and 10 μm (B’”-C””).

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    Article Title: Inhibiting SOX18 with propranolol restores vascular integrity in NR2F2-driven malformations

    doi: 10.1101/2025.04.25.650344

    Figure Lengend Snippet: A Immunostaining of sagittal sections from a CS17 human embryo. Co-expression of NR2F2 (green) and SOX18 (blue) in the gut. scale bars 50 μm. B-B ”,and C-C ” Sections of proband liver (B) and duodenum (C) stained for CD31 (cyan), SOX18 (red arrows), NR2F2 (green arrows), and DAPI (purple). Orange and gold boxes denote zoomed in regions of individual vessels. Orange box represents vessel that shows cells co-expressing both NR2F2 and SOX18 in the same nucleus, whereas the gold box is a vessel with cells either expressing NR2F2 or SOX18. Scale bar = 50μm (B-C) and 10 μm (B’”-C””).

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    Techniques: Immunostaining, Expressing, Staining